Microbiologia

Mostrando 1–12 de 59 resultados

  • AGAR BACILUS CEREUS BASE COM 500G – MERCK

    CEREUS SELECTIVE AGAR BASE MOSSEL (MYP AGAR) MERCK
    RENDIMENTO 21,5g / 450ml H2O
    Embalagem 500g.

    MYP AGAR (mannitol egg yolk polymyxine agar).
    Typical Composition (g/litre)
    Peptone from casein 10.0;
    meat extract 1.0;
    D(-)mannitol 10.0;
    sodium chloride 10.0;
    phenol red 0.025;
    Agar-agar 12.0

    Also to be added (per litre of medium):
    egg-yolk emulsion 100 ml;
    polymyxin B sulfate 100,000 IU = Bacillus cereus Selective Supplement.

    NECESSÁRIO SUPLEMENTO 1098750001 E EMULSÃO GEMA DE OVO.

    Preparation
    Suspend 21.5 g in 450 ml demin. water, autoclave (15 min at 121°C). Cool to about 45 to 50 °C, add 50 ml (this volume can be varied depending on the degree of turbidity desired) of sterile egg-yolk emulsion and the contents of 1 vial Bacillus cereus Selective Supplement, mix. Pour plates.
    pH: 7.2 ± 0.2 at 25 °C.
    The plates (incl. egg-yolk) are evenly turbid and slightly orange
    (red without egg-yolk).

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  • AGAR BAIRD PARKER GRANULADO COM 500G – MERCK

    BAIRD PARKER AGAR GRANULADO MERCK
    RENDIMENTO 61,05g/l STAPHYLOCOCCUS SELECTIVE AGAR BASE
    Embalagem 500g

    Typical Composition (g/litre)
    Peptone from casein 10.0;
    meat extract 5.0; yeast extract 1.0;
    sodium pyruvate 10.0; glycine 12.0;
    lithium chloride 5.0;
    agar-agar 15.0.

    Devera ser adicionado
    Egg-yolk tellurite emulsion 50 ml; if required, sulphamethazine 0.05 g/l.

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  • AGAR BHI ( BRAIN HEART INFUSION) GRANULADO 500GR – MERCK

    BRAIN HEART INFUSION AGAR (BHI) GRANULADO MERCK
    RENDIMENTO 52,0g/L MS8014130089
    EMBALAGEM 500GR

    Typical Composition (g/litre)
    Nutrient substrate (brain extract, heart extract and peptones) 27.5; D(+)glucose 2.0; sodium chloride 5.0; di-sodium hydrogen phosphate 2.5; agar-agar 15.0.

    Preparation
    Suspend 52 g Brain Heart Agar/litre, autoclave (15 min at 121°C). pH: 7.4 ± 0.2 at 25 °C.
    The broth is clear and brown, the agar is clear sometimes slightly opalescent and brown.

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  • AGAR BISMUTH SULFITE GRANULADO MERCK – EMB 500G

    BISMUTH SULFITE AGAR GRANULADO MERCK MS 80141300389
    RENDIMENTO 47,5g/l (SEG. WILSON-BLAIR) SHELF LIFE 1 ANO
    Embalagem 500g

    Mode of Action
    Brilliant green and bismuth largely inhibit the accompanying bacterial flora. Colonies of H2-S-positive salmonellae exhibit blackening due to the formation of iron sulfide. Reduction of bismuth ions to metallic bismuth produces a metallic lustre around the colonies (McCOY 1962).

    Typical Composition (g/litre)
    Meat extract 5.0; peptone from meat 10.0; D(+)glucose 5.0; disodium hydrogen phosphate 4.0; iron(III) sulfate 0.3; brilliant green 0.025; bismuth sulfite indicator 8.0; agar-agar 15.0.

    Preparation and Storage
    Usable up to the expiry date when stored dry and tightly closed at +15 to +25° C. Protect from light.After first opening of the bottle the content can be used up to the expiry date when stored dry and tightly closed at +15 to +25°C. Suspend 47.5 g/litre, mix the resulting precipitate to give a uniform suspension, pour plates to give thick layers (25 ml).
    Do not autoclave.
    pH: 7.6 ± 0.2 at 25 °C.
    The prepared medium is turbid and green in colour.

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  • AGAR BLOOD BASE GRANULADO 500GR – MERCK

    BLOOD AGAR BASE GRANULADO MERCK
    RENDIMENTO 40,0g/l
    EMBALAGEM 500GR

    Typical Composition (g/litre)
    Nutrient substrate (heart extract and peptones) 20.0;
    sodium chloride 5.0:
    agar-agar 15.0

    Also to be added: Blood 50-80 ml.

    Preparation and Storage
    Usable up to the expiry date when stored dry and tightly closed at +15 to +25 °C. Protect from light.After first opening of the bottle the content can be used up to the expiry date when stored dry and tightly closed at +15 to +25 °C. Suspend 40 g/litre, autoclave (15 min at 121 °C), cool to 45-50°C, add 5-8 % defibrinated blood, mix.
    pH: 6.8 ± 0.2 at 25 °C.
    Before adding blood, the prepared medium is clear and yellowish-brown, then blood coloured and not haemolytic.

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  • AGAR BPLS (BRILLIANT GREEN PHENOL RED LAC. SUCROSE) USP EMB 500G – MERCK

    BPLS AGAR BRILLIANT GREEN PHENOL RED LAC. SUCROSE USP MERCK
    RENDIMENTO 51,0g/L
    Embalagem 500g.

    Typical Composition (g/litre)
    Peptone from meat, peptic 5.0;
    peptone from casein 5.0;
    yeast extract 3.0;
    sodium chloride 5.0;
    lactose 10.0;
    sucrose 10.0;
    phenol red 0.08;
    brilliant green 0.0125;
    agar-agar 13.0.

    Preparation and Storage
    Usable up to the expiry date when stored dry and tightly closed at +15 to +25°C. Protect from light.
    After first opening of the bottle the content can be used up to the expiry date when stored dry and tightly closed at +15 to +25°C. Suspend 51 g/litre, autoclave (15 min at 121°C), pour plates. pH: 6.9 ± 0.2 at 25 °C.
    The plates are clear and red-brown.

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  • AGAR BROLACIN (CLED AGAR) GRANULADO COM 500G – MERCK

    BROLACIN AGAR (CLED AGAR) GRANULADO MERCK
    RENDIMENTO 33,0g/l
    EMBALAGEM DE 500G

    BROMOTHYMOL BLUE LACTOSE CYSTINE AGAR, Brolacin Agar ou CLED Agar.
    Embalagem 500g.

    Typical Composition (g/litre)
    Peptones 7.0;
    yeast extract 2.0;
    meat extract 2.0;
    L-cystine 0.128; lactose 10.0;
    bromothymol blue 0.03;
    agar-agar 12.0.

    Preparation and Storage
    Usable up to the expiry date when stored dry and tightly closed at +15 to +25° C. Protect from light .
    After first opening of the bottle the content can be used up to the expiry date when stored dry and tightly closed at +15 to +25° C. Suspend 33 g/litre, autoclave (15 min at 121 °C), p our plates.
    pH: 7.3 ± 0.2 at 25 °C.
    The plates are clear and bluish green

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  • AGAR COLUMBIA BASE 500GR – MERCK

    COLUMBIA AGAR BASE SEG HARM EP/USP/JP MERCK
    RENDIMENTO 42,0g/l
    EMBALAGEM 500GR

    Typical Composition (g/litre)
    Peptrone from casein 10.0;
    peptone from meat 5.0;
    heart extract 3.0;
    extract from yeast 5.0;
    starch 1.0;
    sodium chloride 5.0;
    agar-agar 13.0.

    Preparation and Storage:
    Usable up to the expiry date when stored dry and tightly closed at +15 to +25° C. Protect from light. After first opening of the bottle the content can be used up to the expiry date when stored dry and tightly closed at +15 to +25°C. Suspend 42 g/litre, autoclave (15 min at 121°C). Cool to 45-50 °C before mixing in heat-sensitive additives.
    pH: 7.3 ± 0.2 at 25 °C.
    The plates are clear and yellowish-brown. After blood is added, they are bright red and non-hemolytic

    Preparation of blood agar: Mix 5 ml blood homogeneously with 95 ml sterile culture medium base. Pour plates.

    Preparation of gentamicin blood agar: Mix 100 ml defibrinated sheep blood and 0.11 ml gentamicin solution homogeneously with 900 ml sterile culture medium base. Pour plates.

    Preparation of boiled agar: Add 10 ml blood to 90 ml sterile culture medium base. Heat the mixture in a water bath for about 10 minutes to 80 °C swirling all the time until the medium becomes chocolate brown in colour, pour plates.

    Preparation of lactose milk egg-yolk agar: Dissolve 42 g dehydrated culture medium, 12 g lactose, 1 g agar-agar in 1 litre demineralized water. Mix in 33 ml/litre of a 0.1 % aqueous solution of neutral red, adjust the pH to 7.0 and autoclave (15min at 121 °C). Cool to 45-50 °C, add approximately 35 ml egg-yolk emulsion/litre and 10 g dried milk/litre and mix homogeneously. Pour plates.

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  • AGAR EMB DE LEVINE (EOSIN METHYLENE BLUE LACTOSE AGAR) COM 500G – MERCK

    LEVINE EMB AGAR (EOSIN METHYLENE BLUE LACTOSE AGAR) MERCK
    RENDIMENTO 36,0g/l
    Embalagem 500g.

    The culture medium complies with the recommendations of the APHA Standard Methods for the Examination of Water and Wastewater (1998) and the United States Pharmacopeia XXVI (2003).

    Typical Composition (g/litre)
    Peptone 10.0; lactose 10.0; di-potassium hydrogen phosphate 2.0; eosin, yellowish 0.4; methylene blue 0.065; agar-agar 13.5.

    Preparation and Storage
    Usable up to the expiry date when stored dry and tightly closed at +15 to +25° C. Protect from light. After first opening of the bottle the content can be used up to the expiry date when stored dry and tightly closed at +15 to +25° C. Suspend 36 g/litre, autoclave (15 min at 121 °C), and pour plates.
    pH: 7.0 ± 0.2 at 25 °C.
    The plates are clear and red-brown.
    If cultivating Candida, add 0,1 mg tetracycline hydrochloride/litre after autoclaving and mix homogeneously. The culture medium then is blue.

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  • AGAR HEKTOEN ENTERICO COM 500G – MERCK

    HEKTOEN ENTERIC AGAR MERCK
    RENDIMENTO 75,0g/l MS8014130089
    EMBALAGEM 500GR

    Typical Composition (g/litre)
    Peptones 15.0;
    sodium chloride 5.0;
    yeast extract 3.0; sucrose 14.0;
    lactose 14.0; salicin 2.0;
    sodium thisulfate 5.0;
    ammonium iron(III) citrate 1.5;
    bile salt mixture 2.0;
    bromothymol blue 0.05;
    acidic fuchsin 0.08;
    agar-agar 13.5.

    Preparation and Storage
    Usable up to the expiry date when stored dry and tightly closed at +15 to +25° C. Protect from light.
    After first opening of the bottle the content can be used up to the expiry date when stored dry and tightly closed at +15 to +25° C. Suspend 75 g in 1 litre of demin. water and let soak for 10minutes.
    Gently heat and bring to boil for a few seconds to dissolve the medium completely.
    Do not autoclave.
    If desired, add 15 mg novobiocin per litre to the cooled (50 °C) medium in form of a filter-sterilized solution. Pour plates.
    pH: 7.7 ± 0.2 at 25 °C.
    The plates are clear and blue-green.

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  • AGAR LISINA DE FERRO 500GR – MERCK

    LYSINE IRON AGAR (LIA) GRANULADO MERCK
    RENDIMENTO 32,0g/l
    EMBALAGEM 500GR

    Typical Composition (g/litre)
    Peptone from meat 5.0;
    yeast extract 3.0;
    D(+)glucose 1.0;
    L-lysine monohydrochloride 10.0;
    sodium thiosulfate 0.04;
    ammonium iron(III) citrate 0.5;
    bromocresol purple 0.02;
    agar-agar 12.5.

    Preparation
    Suspend 32 g/litre, dispense into test tubes, autoclave (15 min at 121 °C). Allow to solidify to give agar slants.
    pH: 6.7 ± 0.2 at 25 °C.
    The prepared culture medium is clear and violet.

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  • AGAR M-FC 500GR – MERCK

    M-FC AGAR MERCK
    RENDIMENTO 52,0g/l COLIFORMES FECAIS POR MEMBRANA FILTRANTE
    EMBALAGEM 500GR

    Usado na detecção de Coliformes Fecais pela técnica da membrana filtrante.

    Mode of Action
    In the beginning the faecal coliforms, derived from the intestinal tract of warm-blooded animals, were separated from the nonfaecal coliforms by use of elevated temperature tests, which needed confirmatory MPN procedure in addition. GELDREICH et al. published the development of a Faecal Coliform (FC) Medium for the membrance filtration technique using an incubation temperature of 44.5 °C ± 0.2 °C. m-FC Agar is supplemented with rosolic acid and incubated at 44.5 °C ± 0.2 °C for 24 h. Peptone and yeast extract serve as nutritious source and bile
    salts are added to inhibit accompanying Gram-positive flora. Lactose can be fermented by faecal coliforms at the elevated temperature to form blue colonies on the ready medium (agar base plus rosolic acid), whereas other organisms show grey colonies.

    Typical Composition (g/litre)
    Proteose peptone 5.0;
    tryptose 10.0;
    yeast extract 3.0;
    sodium chloride 5.0;
    bile salts 1.5;
    lactose 12.5;
    methyl blue (formerly aniline blue) 0.1;
    agar-agar 15.0.

    Preparation
    Suspend 52 g in 1 litre of distilled or deionized water and heat to boiling to dissolve completely. Add 10 ml of a 1 % solution of rosolic acid in 0.2 N NaOH. Continue heating for 1 minute with frequent agitation. Do not autoclave! Cool to 45-50 °C. Dispense 4 ml amounts into Petridishes (Æ 50-60 mm) and allow to solidify. Cool to 50 °C and pour plates.
    pH: 7.4 ± 0.2 at 25 °C.
    The plates are clear and blue to violet.

    Experimental Procedure
    Place membrane filter, through which the sample has been filtered, on the surface of the agar. Avoid formatoin of air bubbles between the filter and the agar surface.
    Incubation: 24 hours at 44.5 °C ± 0.2 °C aerobically.

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